Fig. 2

Knockdown of SRSF3 suppresses the expression of BRCA1, BRIP1 and RAD51. a Regular PCR amplification of cDNA fragments of BRCA1, BRIP1, RAD51, SRSF3 and GAPDH. b Relative quantitation of BRCA1, BRIP1, RAD51 and SRSF3 expression determined by qPCR analysis. Shown are the results of three independent experiments (mean ± s.d.). * indicates p < 0.01 for comparisons between samples treated with and without Doxy. c Left: Western blotting results of whole cell lysates of A2780 subline cells treated with or without Doxy. Right: Quantitation of the western blotting results. Results in (a), (b) and (c) were obtained from cells treated with or without Doxy for 3 days. d Time course of BRCA1, BRIP1, RAD51 and SRSF3 expression at mRNA levels determined by qPCR. Day 0 represents cells that were not treated with Doxy. Two independent experiments were performed and produced similar results. Shown are the results of one experiment (mean ± s.d. of triplicate PCR reactions). e Time course of BRCA1, BRIP1, RAD51 and SRSF3 expression at protein levels. Left: Western blotting results; Right: Quantitation. f Time course of apoptotic cells