Fig. 1
From: Retinoic acid-induced CHD5 upregulation and neuronal differentiation of neuroblastoma
Neuronal differentiation of SY5Y cells with 13cRA treatment. (a) Representative phase contrast images showing SY5Y cell morphology change with or without 13cRA treatment (10 μM, 12 days). Multiple long neurites were observed in the presence of 13cRA. (b) Immunofluorescence of SY5Y cells with or without 13cRA treatment. Representative immunofluorescence images obtained when cells were treated with 13cRA and stained with neuronal markers tyrosine hydroxylase (TH), synaptophysin (SYP), and βIII-Tubulin. Control cells did not show significant staining with TH or SYP, but there was some βIII-Tubulin staining