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Fig. 2 | Molecular Cancer

Fig. 2

From: CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA

Fig. 2

HUVECs characterization after exosomes treatment: a RT-PCR analyses for VEGF, VEGF-R and ICAM1 were done on HUVECs 18 h after treatment with CD90+ or Huh7-derived exosomes (5 μg/ml). ΔΔct expressed as fold of induction (FOI) compared with control (untreated cells). ***p < 0.001; *p < 0.05. b Left panel: ELISA for VEGF released by HUVECs 18 h after treatment with CD90 + exo or Huh7exo. Untreated cells were used as control. *p < 0.05. Middle-right panels: Tubulogenesis analysis. Phase contrast micrographs (20×) and quantification of matrigel assay expressed as length of cable as arbitrary unit. c FACS analysis for ICAM-1 on HUVECs 18 h after treatment with Huh7exo or CD90 + exo, respectively. d Adhesion capacity. Left panel: Phase contrast micrographs (20×) showing the adhesion of CD90 + cells on HUVEC monolayer pre-treated with Huh7exo or CD90 + exo. Right panel: Quantification of adhesion established by counting the number of adherent CD90 + cells (violet) per field; *p < 0.05

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Molecular Cancer

ISSN: 1476-4598