Fig. 1
Na,K-ATPase subunits in medulloblastoma and CGP cells. a. Na,K-ATPase α1- and β1-subunit expression in cerebellum from 6 month old WT C57BL6/J mice (WT) and tumors from age-matched Smo/Smo mice. An immunoblot for GAPDH confirmed equal loading of protein. b. Na,K-ATPase α1-subunit and β1-subunit mRNA levels in WT and medulloblastoma cerebellum normalized to beta-2 microglobulin. For both α1- and β1-subunit the difference between WT and medulloblastoma cerebellum is statistically significant (p < 0.005) (n = 3). c. Primary CGP cells isolated from C57BL/6 J mice were cultured in vitro for 3, 6, 9 and 12 days and then lysed for immunoblotting with indicated antibodies. α-tubulin was used as a loading control. d. mRNA levels of α1- and β1-subunit during differentiation of CGP cells cultured in vitro and normalized to beta-2 microglobulin