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Fig. 2 | Molecular Cancer

Fig. 2

From: Long non-coding RNA TUG1 is up-regulated in hepatocellular carcinoma and promotes cell growth and apoptosis by epigenetically silencing of KLF2

Fig. 2

Effects of knockdown of TUG1 on HCC cells viability and apoptosis in vitro. a,b The TUG1 expression level was determined by qPCR when HepG2 and Hep3B cells transfected with si-TUG1. c,d MTT assays were used to determine the cell viability for si-TUG1-transfected HepG2 and Hep3B cells. Values represented the mean ± s.d. from three independent experiments. e,f Colony-forming assays were conducted to determine the proliferation of si-TUG1-transfected HepG2 and Hep3B cells. g,h Flow cytometry assays were performed to analysize the cell cycle progression when HCC cells transfected with si-TUG1 24 h later. The bar chart represented the percentage of cells in G0/G1, S, or G2/M phase, as indicated. i,j Flow cytometry assays were performed to analysis the cell apoptosis when HCC cells transfected with si-TUG1 48 h later. k,l PARP cleavage protein via western blot after TUG1 depletion. *P < 0.05, **P < 0.01

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Molecular Cancer

ISSN: 1476-4598