Invasion of ovarian cancer cells is induced byPITX2-mediated activation of TGF-β and Activin-A

Table 2 The sequence, respective amplicon size and Tm of the oligonucleotide primers used in Q-PCR

Gene Name	Forward primer (5′- 3′)	Reverse primer (5′- 3′)	Amplicon size (bp)	Tm (°C)
18S rRNA	GATTCCGTGGGTGGTGGTGC	AAGAAGTTGGGGGACGCCGA	134	60
POLR2A	TGGACCCACCGGCATGTTCT	GCCCCTGGGGTCATTCCACT	141	60
CLDN4	GGCTGCTTTGCTGCAACT	CAGAGCGGGCAGCAGAATAC	110	60
CLDN7	GTGGCAGATGAGCTCCTATGC	CATCCACAGCCCCTTGTACA	80	60
DSM	GCAGCAAAGGGCGGAGAT	TGTTAATGTGCTGCTCCACTGA	80	60
VIM	ACACCCTGCAATCTTTCAGACA	GATTCCACTTTGCGTTCAAGGT	80	60
FN1	CCTTCATGGCAGCGGTTT	AGCGTCCTAAAGACTCCATGATCT	94	60
PITX2	CGCGAAGAAATCGCTGTGT	CGACGATTCTTGAACCAAACC	78	58
TGFB1	GTGACAGCAGGGATAACACACTG	CATGAATGGTGGCCAGGTC	80	60
TGFB2	GCTGAGCGCTTTTCTGATCCT	CGAGTGTGCTGCAGGTAGACA	80	60
TFGB3	CACCACAACCCTCATCTAATCCT	CCTGGCCCGGGTTGTC	100	60
SNAI1	TCGGAAGCCTAACTACAGCGA	AGATGAGCATTGGCAGCGAG	140	60
SNAI2	ATGAGGAATCTGGCTGCTGT	CAGGAGAAAATGCCTTTGGA	119	60
CDH1	GTCACTGACACCAACGATAATCT	TTTCAGTGTGGTGATTACGACGTA	100	60
MMP9	ACCTCGAACTTTGACAGCGAC	GAGGAATGATCTAAGCCCAGC	113	60
INHBA	GTGAGTGCCCGAGCCATATAG	CATGCGGTAGTGGTTGATGACT	80	60
CDH2	CCATCAAGCCTGTGGGAATC	GCAGATCGGACCGGATACTG	76	60
ZEB1	CAATGATCAGCCTCAATCTGCA	CCATTGGTGGTTGATCCCA	117	60
ZEB2	AAGCCCCATCAACCCATACAAG	AAATTCCTGAGGAAGGCCCA	124	60

ISSN: 1476-4598