Fig. 7
Western blot analyses on expression of CD91, TLR2 and TLR4 proteins on various TCL-activated DCs. Test 4 T1 cells were dispensed in 6-well plates (3 × 105cells/well) and incubated with vehicle, SK or Dox at 5 μg/ml for 24 h. Some sets as replicates collected from SK-TCL samples were depleted for specific ICD molecules, i.e., HSP70, CRT or HMGB1, using an antibody-mediated pull-down procedure (see Materials and Methods). Different ICD molecule-depleted SK-TCL samples were then compared for their stimulatory activity on expression of CD91, TLR2 and TLR4 on TCL-pulsed DCs. Beta-actin was used as a loading control. The results represent the similar trend of data obtained from three independent experiments