Table 1 Real-time PCR primer sequences
Gene | Sequence (5′–3′) | Annealing temp (°C) | ID | |
|---|---|---|---|---|
FASN | Sense | GGAATGGGAAGACACCTATGGA | 62 | GenBank NM_004104.4 |
Antisense | AGAGAGAGCTCAGATACGTTGAC | |||
PPARG | Sense | TCTCTCCGTAATGGAAGACC | 62 | GenBank L40904.2 |
Antisense | GCATTATGAGACATCCCCAC | |||
ACTB | Sense | CCATCATGAAGTGTGACGTTG | 62 | GenBank NM_001101.3 |
Antisense | ACAGAGTACTTGCGCTCAGGA | |||
PPARG Hydrolysis Probe | Sense | GATGTCTCATAATGCCATCAGGTT | 60 | RTPrimerDB ID: 5471 |
Antisense | GGATTCAGCTGGTCGATATCACT | |||
Probe | CCAACAGCTTCTCCTTCTCGGCCTG | |||
ACTB Hydrolysis Probe | Sense | TCCTTCCTGGGCATGGAG | 60 | RTPrimerDB ID: 2418 |
Antisense | AGGAGGAGCAATGATCTTGATCTT | |||
Probe | CCTGTGGCATCCACGAAACTACCTTC | |||