Fig. 4

Scrib is dispensable for the maintenance of normal adult epidermal homeostasis. a PCR analysis of genomic DNA to detect Wt (290 bp), floxed (390 bp) and recombined (550 bp) Scrib alleles in Scrib ^+/+, Scrib ^+/fl and Scrib ^fl/fl adult dorsal epidermis (n = 3). b qRT-PCR for Scrib mRNA confirmed a significant reduction in Scrib ^+/fl and Scrib ^fl/fl adult dorsal epidermis compared to Scrib ^+/+ controls (*p ≤ 0.0001, unpaired t-test, n = 3). c Representative H&E (n = 10), Scrib IF (Scrib = green, DAPI = blue), PCNA IHC and CC3 IHC images of Scrib ^+/+, Scrib ^+/fl and Scrib ^fl/fl adult dorsal epidermis (n = 3). Scale bar = 50 μm (insert 1–3: scale bar = 10 μm). Arrow represents positive CC3 staining. Quantitation of nuclear PCNA (d) and CC3 (e) positive cells in adult dorsal epidermis shows no significant difference in Scrib-deficient epidermis compared to Scrib ^+/+ controls (p ≥ 0.6188, unpaired t-test, n = 3). Adult mice were 100 day old. Error bars = SD