Fig. 6

Scrib depletion promotes DMBA/TPA-induced epidermal tumor progression. a Representative H&E images of mouse epidermis displaying cutaneous hyperplasia, benign papilloma formation and invasive SCC following long-term DMBA/TPA treatment (scale bar = 1 mm). b Percentage phenotype incidence at end-point (P = 0.0117, two-way ANOVA, n = 12–16) in Scrib ^+/+ , Scrib ^+/fl and Scrib ^fl/fl mice that have undergone long-term DMBA/TPA treatment. Scrib ^+/+ Scrib ^+/fl and Scrib ^fl/fl respective phenotype incidence; hyperplasia (32.1 %, 9.7 % and 10.7 %), benign papillomas (64.2 %, 75.8 %, 64.3 %) and invasive SCC (3.8 %, 14.4 % and 25 %) . c IHC images for BrdU, CC3 and γH2AX (scale bar = 50 μm, n = 3) and quantitation of the number of (d) BrdU, (e) CC3 and (f) γH2AX positive cells in Scrib ^+/+, Scrib ^+/fl and Scrib ^fl/fl mice that have undergone long-term DMBA/TPA treatment (% per 40x magnification field, *p ≤ 0.0486, unpaired t-test, error bars = SD, n = 3)