Fig. 2

GATA4 and GATA6, transcription factors of BMP4, are miR-200 target genes. a Luciferase assay of Bmp4 promoter activity. 393P cells were co-transfected with GATA4 or GATA6 expression vector and the Bmp4 promoter reporter. Results were normalized by using a Renilla luciferase vector (pRL-TK). Mean + SD, n = 3; p, two-tailed Student’s t-test. b, c Luciferase assay of Gata4 and Gata6 3′-UTR activity. 344SQ cells were co-transfected with control (con) or miR-200 mimics and 3′-UTR reporter constructs. Results were normalized by using a firefly luciferase vector (pGL3-con). ZEB1 3′-UTR construct was used as a positive control. Mean + SD, n = 3; p, two-tailed Student’s t-test. d qRT-PCR of Gata4, Gata6, and Zeb1 in 344SQ cells transfected transiently with control (con) or miR-200b mimic. The expression levels were normalized to those of the control group (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. e Bmp4 qRT-PCR in 344SQ_miR-200 cells transfected with GATA4 or GATA6. The expression levels were normalized to that of vector-transfected cells (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test