Fig. 5
From: BMP4 depletion by miR-200 inhibits tumorigenesis and metastasis of lung adenocarcinoma cells
Non-muscle myosin is a downstream target of BMP4. a Heatmap of differentially expressed genes (fold change ≥1.5, p value <0.05) identified by mRNA microarray of 344SQ-NTC and Bmp4-KD (#2) cells. Yellow: increased expression; blue: decreased expression. b qRT-PCR of 29 selected genes to confirm the microarray data. Mean + SD, n = 3; p, two-tailed Student’s t-test. c qRT-PCR of Bmp4 and Myh10 in 344SQ-NTC and Bmp4-KD cells (#2 and #3). The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. d qRT-PCR of Myh10 in 344SQ-NTC and Myh10-KD cells (#3 and #4). The expression levels were normalized to those of 344SQ-NTC (=1.0). Mean + SD, n = 3; p, two-tailed Student’s t-test. e, f Matrigel 3-D culture of 344SQ-NTC and Myh10-KD cells (KD#3 and #4; E), and 344SQ cells in the presence or absence of blebbistatin (Bleb, 25 μM; F). Phase contrast microscope images of 3-D acini were taken 12 days after cell seeding