Fig. 2

Time course of changes in expression of miR-92a, c-Myc, Drosha and p57 following butyrate treatment. HCT116 human colon cancer cells were treated with 2 mM butyrate for up to 24 h. Cells were harvested for analysis at 1, 2, 4, 8, 16, and 24 h after treatment. a The abundance of pri-miR-17-92a, pre-miR-92a, and mature miR-92a was measured using qPCR. Bars represent means ± SEM. n = 3. b Protein levels of c-Myc, Drosha and β-actin were analyzed by immunoblotting. The image shown is representative of three individual experiments. c The abundance of c-Myc mRNA was measured using qPCR. Bars represent means ± SEM. n = 3