Fig. 7

Over-expression of miR-92a attenuates butyrate-induced p57 expression by blocking p57 translation. a HCT116 human colon cancer cells were treated with 2 mM butyrate for up to 24 h. Cells were harvested for analysis at 1, 2, 4, 8, 16, and 24 h after treatment. Protein levels of p57and β-actin were analyzed by immunoblotting. The image shown is representative of three individual experiments. b HCT116 cells were transfected with miR-92a mimetics or control miRNA (miR-C) using Lipofectamine 2000 then treated with 2 mM butyrate for 24 h prior to harvest. Cells without butyrate treatment (UNTD) or treated with Lipofectamine 2000 (Lipo) were analyzed as controls. The abundance of miR-92a was measured using qPCR. c p57 mRNA levels were measured using qPCR. d Protein levels of p57 and β-actin were analyzed by immunoblotting. The image shown is representative of four individual experiments. e Normalized densitometric values of p57 protein levels. Bars represent means ± SEM. *P < 0.05, n = 4