Fig. 6

Loss of ACVR2A in vivo is associated with pAkt downregulation in intestinal tumors. Loss of ACVR2A leads to pAkt downregulation and p21 upregulation in ACVR2A knockout KO mice. ACVR2A wild type (wt) and ACVR2A KO mice were used in a DSS/AOM intestinal cancer model. Mice were injected intraperitoneally with AOM and after 5 days were given 3 cycles of DSS. Lysates from normal (a) and 10.1186/s12943-015-0456-4 intestinal tumor tissue (b) were probed for p21, pan-Akt, and pAkt expression via Western blot. Tumor and normal tissue from four different mice from each group of ACVR2A wt and ACVR2A KO mice is shown. c Loss of ACVR2A is associated with a decrease in pAkt and an increase of p21, which is most pronounced in intestinal tumor tissue. Immunobloy signal was quantified by densitometry and the ratio of p21 to pAKT expression was determined