Fig. 4
From: PKM2 regulates neural invasion of and predicts poor prognosis for human hilar cholangiocarcinoma
Knockdown of PKM2 expression led to inhibition of NI of HC cells in mice in vivo. a and b, in situ tumor xenograft models were used to assess the inhibitory effect of PKM2 knockdown (a) compared with in a negative control (NC) (b) as determined using anatomical (left) and ultrasonic (right) methods. c and d, tumors were weighed (c) and survival durations were calculated (d) at the end of the experiment. Cum, cumulative. e, histological (E1) and immunohistochemical (E2) assays demonstrated NI of tumors with high PKM2 expression (control group; E3), whereas NI of tumors with low PKM2 expression was rare (PKM2-knockdown group; E4). Original magnification, ×200. f, graphical representation of the rate of NI of HC in the PKM2-knockdown and control groups. g, graphical representation of the intensity of PKM2 staining in the PKM2-knockdown and control groups