Fig. 5From: PKM2 regulates neural invasion of and predicts poor prognosis for human hilar cholangiocarcinomaCorrelation between PKM2 expression and SDC2 expression in HC cases. QBC939 cells were transiently transfected with control shRNA or PKM2 shRNA. a and b, the expression of several genes associated with NI and metastasis was measured using reverse transcription-polymerase chain reaction (a), and the level of PKM2 and SDC2 expression was determined using Western blotting (b). GAPDH, glyceraldehyde-3-phosphate dehydrogenase. c, graphical representation demonstrating a significant correlation between PKM2 expression and SDC2 expression in human HC tissues. d, co-localization of PKM2 and SDC2 in the consecutive sections of HC tissue. e, co-localization of PKM2 and SDC2 in the consecutive sections of HC tissue with NI. Original magnification, ×40 (left) and × 400 (right)Back to article page