Fig. 4

Effects of short- and long-term TQ treatment in macrophages isolated from ascites fluid. a Overall number of mononuclear cells increased in ascites collected from mice treated with thrice weekly 20 mg/kg TQ or vehicle (Veh) for 10 or 30 days, as quantified in differential cell counts of H&E-stained cytospin slides. b QPCR measurement of mRNA levels of established M2 (mannose-receptor and IL-10) and M1 (CCL3) macrophage markers. Values were expressed relative to corresponding GAPDH levels. c Ratio of M2 (mannose-receptor and IL-10) to M1 (CCL3) markers expressed as fold TQ-induced change in harvested peritoneal macrophages. *p < 0.01 compared to vehicle; #p < 0.01 compared to all other tumor types; *p < 0.01, both Mann–Whitney test