Fig. 8

Clodronate preferentially depletes non-M2 macrophages in mice co-treated with TQ. a IF analysis of expression of the macrophage markers F4/80 (green) and arginase-1 (red) in ascites harvested from mice treated with weekly clodronate/empty liposome (EL) and thrice weekly vehicle or 20 mg/kg TQ treatment for 30 days. Ascites contains of mixture of F4/80-positive macrophages and F4/80-negative cells (for example, tumor cells). DAPI-stained nuclei are in blue. b Quantification of the percentage of cells staining positive for F4/80, and for both F4/80 and arginase-1. Values were determined from 5 representative fields at high power (×40). *p < 0.01 relative to vehicle; all p values determined by Mann–Whitney test