Fig. 7

Knockdown of GADD45α expression neutralized the effect of the miR-362-5p inhibitor. a Proliferation of BV173 and K562 cells transfected with inhibitor control (inhibitor-ctrl) and siGADD45α scrambled oligonucleotide (si-ctrl), inhibitor-ctrl and siGADD45α, inhibitor and si-ctrl, or inhibitor and siGADD45α was determined using the CCK-8 assay at the indicated times. The values represent the means ± SD of three replicates. b BV173 and K562 cells (in the absence or presence of 5 μg/ml Ara-c) were stained with PI and Annexin V after cotransfection, as described above. Early and late apoptotic cells are shown in the right quadrant. *P < 0.05,**P < 0.01