Fig. 3

Effect of ATP and AZ10606120 on BrdU incorporation in PDACs and HPDE cells. Data for each cell line are given in one panel and filled black symbols show the effect of different concentrations of added (exogenous) ATP (100 μM, 1 mM and 5 mM), or control (no exogenous ATP added), on BrdU incorporation in all cell lines after 60 h. The colored symbols show the effect of ATP and control in combination with the allosteric inhibitor AZ10606120 (10 μM), which was added 1 h before ATP. The results were normalized to 1 % serum control (100). The graphs show data from three to six experiments (mean ± SEM); where each run was carried out in triplicates. Significant differences P < 0.05 (*, #) and P < 0.001 (**) from the respective 1 % serum control (*, **) and with/without inhibitor (#) are indicated