Fig. 1

NHL cell lines are resistant to AZD1208 treatment. a Cells were seeded into duplicate wells of 6-well plates and treated with DMSO, 1 μM, 5 μM or 10 μM AZD1208 for indicated times. The medium was replaced every day and cell number was measured by MTT assay. The means and SD of at least two independent experiments, conducted in duplicate wells of six-well plates, are plotted. b, c Cells were treated like in (a). Aliquots were harvested every second day and analysed by western blot. d Cells were treated with 1 μM, 2.5 μM, 5 μM, 7.5 μM or 10 μM AZD1208 or a respective amount of DMSO for three days. Then, protein expression was evaluated by western blot