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Fig. 6 | Molecular Cancer

Fig. 6

From: BALR-6 regulates cell growth and cell survival in B-lymphoblastic leukemia

Fig. 6

SP1 transcriptome is modulated by BALR-6. a Confirmation of SP1 and CREB1 expression in RS4;11 microarray samples, as well as Reh knockdown cell lines. Normalized to ACTIN. (b) SP1 and CREB1 transcript level increase correlates with overexpression of BALR-6 in Nalm-6 cells (top) and 70Z/3 cells (bottom). Quantitation by qRT-PCR, normalized to ACTIN (Nalm-6 cells) or L32 (70Z/3 cells). c Schematic depicting location of cloned promoter sequences in the pGL4.11 vector system for luciferase assays. d Transcriptional activity at CREB1 (left) and p21 (right) promoter regions upon SP1 and/or BALR-6 overexpression, as measured by luciferase activity. e Quantitation of overexpression in luciferase assays (as seen in (d)) by qRT-PCR of respective transcripts, normalized to ACTIN. Evaluations were made using a two-tailed T-test, p < 0.05 (*); p < 0.005 (**); p < 0.0005 (***). luc2p, synthetic firefly luciferase

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