Fig. 2
From: PKM2 promotes tumor angiogenesis by regulating HIF-1α through NF-κB activation
PKM2 depletion is associated with impaired proliferation and augmented apoptosis in tumor cells. a 15 × 103 Capan1, BxPC3 and Panc1 pancreatic cancer cells transiently transduced either with a PKM2-specific shRNA or a non-coding shRNA (shScr) were plated in 24 well dishes and allowed to grow for the next 120 h. Cell number was quantified every 24 h. Error bars represent mean +/- SEM of two independent experiments performed in triplicate. b lysates of transiently transduced cancer cells were subjected to western blot analysis with PKM2 antibody as indicated. β-actin was used as loading control. c various pancreatic cancer cells were stably transduced with two PKM2-specific shRNAs (shPKM2 #2 or shPKM2 #4) or a non-targeting shRNA (shScr). Lysates were prepared and subjected to SDS-PAGE analysis. Membranes were incubated with PKM2 and cleaved PARP antibodies. d PaTu2 pancreatic cancer cells stably infected with PKM2-specific shRNAs or a non-targeting shRNA were subjected to annexin V / propidium iodide staining and subsequent flow cytometry. The experiment was performed in triplicate and the data is shown as mean +/- SEM. One of the two experiments is shown