Skip to main content

Advertisement

Fig. 2 | Molecular Cancer

Fig. 2

From: Key role of MEK/ERK pathway in sustaining tumorigenicity and in vitro radioresistance of embryonal rhabdomyosarcoma stem-like cell population

Fig. 2

Expression of stem cell phenotype in other cell lines and effects of U0126. a and d Representative microphotographs of TE671 or RH30 cells in SC medium after 7 days of incubation in the absence (rhabdospheres, SC-mSPH) or in the presence of 40 μM U0126 (SC-m + U) (Bar = 100 μm). b and e Histograms of percentage of CD133 and CXCR4 positive cells determined by FACS analysis. Values represent the mean ± SD of 3 independent experiments. Statistical significance is indicated (*p < 0.05). c Western blot analysis of protein lysates from TE671 in SC medium without (SC-mSPH) or with different concentrations of U0126 (10–40 μM) (SC-m + U) at 7 days. f Western blot analysis of protein lysates from RH30 cells in SC medium without (SC-mSPH) or with 40 μM of U0126 (SC-m + U) at the times indicated. The expression levels of ERK1/2-PO4 and ERK1/2 were analyzed. ERK1/2-PO4 bands were quantified by densitometry and compared with ERK1/2

Back to article page