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Fig. 1 | Molecular Cancer

Fig. 1

From: ERK mediated upregulation of death receptor 5 overcomes the lack of p53 functionality in the diaminothiazole DAT1 induced apoptosis in colon cancer models: efficiency of DAT1 in Ras-Raf mutated cells

Fig. 1

Apoptosis induction by DAT1 in p53 mutated cell lines a. Indicated cell lines were treated with DAT1 for a period of 24 h and stained with DAPI. b. Apoptosis was quantitated by counting the number of cells with condensed chromosomes and percentage was calculated as compared to the total number of cells. Cells were counted from five different fields. Values expressed in the graph are the average values from three independent experiments c. Cells were treated with DAT1 for the indicated time periods and caspase 8 and 3 activation were checked using western blotting. Band intensities determined by Biorad Quantity one software were normalized with the levels of beta actin and fold increase was quantified with respect to the untreated ones

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Molecular Cancer

ISSN: 1476-4598