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Fig. 7 | Molecular Cancer

Fig. 7

From: ERK mediated upregulation of death receptor 5 overcomes the lack of p53 functionality in the diaminothiazole DAT1 induced apoptosis in colon cancer models: efficiency of DAT1 in Ras-Raf mutated cells

Fig. 7

Measurement of apoptosis in tumour tissues. A. Tumours bearing HCT116 or HCT116 p53 −/− cells were excised after 28 days of injection, fixed in 4 % paraformaldehyde and preserved in 30 % sucrose and cryosectioning was performed. a,c. Tunnel assay. Brown color (DAB staining) indicates the apoptotic cells. b,d. Immunuhistochemical staining of active caspase 3 in HCT116 and HCT116 p53 −/− . Alkaline phophatase detection method was used to visualize the antibody bound cells. B. The area showing blue/brown colour were quantified by Image J software and plotted. p values for the differences among treatment values and control values were obtained by unpaired t test,** stands for p ≤ 0.001 and * for p ≤ 0.01. At least five fields were analysed

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Molecular Cancer

ISSN: 1476-4598