Fig. 4

Real time PCR analysis of Rad51C-ATXN7 fusion gene Variant 2 expression in LS-174 T and RKO colorectal tumor cells pre and post 5-Azacytidine treatment. The LS-174 T and RKO cells were treated with 5-Azacytidine at dose of 5 μM for 72 h. The total RNA was isolated from pre and post 5-Azacytdine treated LS-174 T and RKO colorectal tumor cells and reverse transcribed to cDNA. The cDNA was then used as template for Rad51C-ATXN7 fusion Variant expression analysis using Taqman real time PCR. The analysis showed 3.51 fold increase in relative expression of RNA for Variant 2 in comparison to untreated control in LS-174 T cells. The RKO cells showed only 1.2 fold mild increase in relative expression of RNA for Variant 2 in comparison to untreated control