Fig. 1

Immunofluorescence detection of the ligand and mediator of hedgehog (Hh) signaling in a culture of pancreatic cancer cells. GLI1: green, sonic hedgehog (SHH): red, DAPI: blue. Scale bar: 100 μm. The experiment was repeated three times and the representative results were indicated. Negative controls, the staining only with secondary antibodies, are depicted in Additional file 1: Figure S1A