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Fig. 7 | Molecular Cancer

Fig. 7

From: Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis

Fig. 7

ITSN-1s impairs mSos1-Eps8 leading to deactivation of Rac1. a Representative PAK/Rhotekin-GST pull-down assays applied on A549 and A549 + ITSN-1s cell lysates followed by WB with Rac1, Cdc42 and RhoA Abs. b Data are shown as percentage of control. Total Rac1, Cdc42, RhoA and GTP/GDP loaded lysates were uses as control. c, d A549 and A549 + ITSN-1s cell lysates were subjected to IP with mSos1 Ab, followed by WB with Rac1 (c, upper panel) and Eps8 (d, upper panel) Abs. The lower panels in c and d illustrate the Rac1, mSos1 and Eps8 protein levels in A549 and A549 + ITSN-1s cell lysates. Mouse IgG was used as control (not shown). e Densitometry of Eps8-mSos1 interaction expressed as percentage of control. All data are representative of 3 independent experiments performed under identical experimental conditions

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Molecular Cancer

ISSN: 1476-4598