Fig. 2
miR-326 is a PI3 kinase regulated miRNA and is downregulated in glioblastoma. a. Transcript levels of miR-326 in U87 and U251 cells upon 24 h of LY294002 treatment in the miRNA-microarray. b, c. Transcript levels of miR-326 in U87 and U251 cells upon PI3 kinase pathway inhibition by LY294002 treatment (50 μM). The transcript levels were assayed at 12 and 24 h time points by real time quantitative PCR. d, Scatter plot depicting the downregulation of miR-326 in GBM in the TCGA dataset as compared to control brain samples. e, Scatter plot depicting the downregulation of miR-326 in GBM in the lab dataset (microarray), each dot represents one sample and for each tumour sample fold change is calculated as difference in the expression value of the miRNA over the average of its expression values in the control brain samples. f, Scatter plot depicting the Log2 transformed miR-326 expression values obtained from qRT-PCR analysis in an independent cohort of GBM samples as compared to control brain samples. g, Log2 transformed miR-326 expression values obtained from qRT-PCR analysis in GBM cell lines LN18, U373, T98G, LN229, U343, SVG, IHA, U138, U251 and U87. For each GBM sample or cell line, fold change in miRNA expression is calculated over its mean expression in control brain samples. p values were calculated by student’s t-test and the symbols are indicated. (*) p ≤ 0.05; (**) p ≤ 0.01; (***) p ≤ 0.001