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Fig. 4 | Molecular Cancer

Fig. 4

From: Lipocalin2 suppresses metastasis of colorectal cancer by attenuating NF-κB-dependent activation of snail and epithelial mesenchymal transition

Fig. 4

LCN2-mediated inhibition of metastasis/invasion in CRC. a, b Migration assay of corresponding cells and quantification of cell migration was measured with microplate reader at 570 nm of OD value. (n = 3, mean ± SD, scale bars, 100 μm). (C,D) Wound-closure assays of LCN2-overexpressing and LCN2-knockdown cells and corresponding vector control cells and quantification of the fold change of average migrated distance (48 h/0 h) of wound healing assays (n = 3, mean ± SD, scale bars, 100 μm). e, f Invasion assay of corresponding cells and quantification of cell invasion was measured with microplate reader at 570 nm of OD value. (n = 3, mean ± SD, scale bars, 100 μm). g Metastatic tumor nodules in the lungs of nude mice after injecting LCN2-overexpressing cells and control cells and quantification of the number of lung metastatic foci per section (n = 5 mice/group, P = .043). h Hematoxylin and Eosin (H.E.) staining of lung metastases of nude mice with corresponding cells (scale bars, 500 μm). * P < .05, ** P < .01, *** P < .001

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Molecular Cancer

ISSN: 1476-4598