Fig. 4
Effects of miR-138-5p and Survivin on the growth of bladder cancer cell xenografts in mice. (a) Overview of the experimental design. T24 cells were infected with either a control lentivirus or a lentivirus containing a plasmid that overexpressed miR-138-5p and then transfected with either a control plasmid or a BIRC5 overexpression plasmid. Each group of T24 cells (2 × 106 cells per 0.1 mL) was subcutaneously injected into 6-week-old nude mice (5 mice per group), and tumor growth was evaluated on day 28 after cell implantation. (b) and (c), Representative images of the injected mice and excised tumors in the different groups. (d), Western blotting analysis of Survivin in tumors from injected mice. (e)-(h), Quantitative analysis of 4 parameters in tumors from the injected mice. (e), Quantitative analysis of the tumor weight, (f), Quantitative RT-PCR analysis of miR-138-5p. (g), Quantitative analysis of Survivin protein levels. (h), Quantitative analysis of Survivin mRNA levels. (I)-(K), H&E-stained sections and immunohistochemical staining for Ki-67 and Survivin in the tumors from injected mice. (i): representative images; (j)-(k): quantitative analysis (J. Ki-67-positive cells; K. Survivin density). *p < 0.05; ** p < 0.01; *** p < 0.005