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Fig. 1 | Molecular Cancer

Fig. 1

From: Aryl hydrocarbon receptor/cytochrome P450 1A1 pathway mediates breast cancer stem cells expansion through PTEN inhibition and β-Catenin and Akt activation

Fig. 1

Constitutive expression of CYP1A1 and CYP1B1 in mammospheres vs adherent breast cancer cell lines. The basal mRNA expression of CYP1A1 and CYP1B1 of human breast cancer cell lines; MCF-7 (a), MDA-MB231 (b), SKBR-3 (c), HS578T (d), and T47D (e) were determined by RT-PCR normalized to β-ACTIN housekeeping gene. Duplicate reactions were performed for each experiment and the values are presented as mean ± SEM (n = 6). *; p < 0.05 compared to corresponding adherent cells. f Mammosphere and adherent populations of MCF-7 cells were stained with primary antibodies against CYP1A1 (green) and ALDH (magenta) followed by secondary antibodies and DAPI (red). Thereafter, the constitute CYP1A1 and ALDH proteins localization was conducted by Immunofluorescence assay. Each sample was stained in triplicate for each antibody

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Molecular Cancer

ISSN: 1476-4598