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Fig. 2 | Molecular Cancer

Fig. 2

From: Aryl hydrocarbon receptor/cytochrome P450 1A1 pathway mediates breast cancer stem cells expansion through PTEN inhibition and β-Catenin and Akt activation

Fig. 2

Effects of AhR activation on breast cancer mammospheres formation. MCF-7 cells were trated with indicated concentrations of TCDD and DMBA for 72 h. Thereafter, cells were trypsinized and viable 1000 cells/100μL were seeded into ultralow attachment plates in serum-free mammary epithelium basal medium, supplemented with B27, EGF, insulin, ABM, and hydrocortisone. Cells grown in these conditions as non-adherent spherical clusters (mammospheres) were allowed to grow for 7 days. The mammosphere cell numbers and size were determined by Evos® transmitted light microscope (Life Technologies Co., Grand Island, NY). Duplicate measurment were performed for each experiment and the values represent mean of fold change ± SEM (n = 3). *; p < 0.05 compared to control

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Molecular Cancer

ISSN: 1476-4598