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Fig. 3 | Molecular Cancer

Fig. 3

From: Aryl hydrocarbon receptor/cytochrome P450 1A1 pathway mediates breast cancer stem cells expansion through PTEN inhibition and β-Catenin and Akt activation

Fig. 3

Effects of AhR/CYP1A1 activation on breast CSCs marker ALDH in vitro and in vivo. a MCF-7 cells were treated for 72 h with increasing concentrations of TCDD (0.1, 1, 10 nM) and DMBA (1.25, 2.5, and 5 μM). b-d Three human breast cancer cells; MCF-7, HS587T, and T47D cells were treated for 72 h with TCDD 10 nM and DMBA 5 μM. Pelleted cells were then incubated with ALDH substrate in the presence and absence of DEAB, ALDH inhibitor. Thereafter, percentage of ALDH+ cells were determined by flow cytometry. Duplicate reactions were performed for each experiment. e-f Twenty virgin female Balb/c mice were injected IP with either corn oil (vehicle) or single dose of DMBA 30 mg/kg. Two months later, mammary gland tissues were collected and stained with H/E for histopathology (e) or with antibody against CYP1A1 and ALDH1/2 for IHC assay (f)

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Molecular Cancer

ISSN: 1476-4598