Fig. 4From: Aryl hydrocarbon receptor/cytochrome P450 1A1 pathway mediates breast cancer stem cells expansion through PTEN inhibition and β-Catenin and Akt activationEffects of AhR/CYP1A1 inhibition on breast CSCs marker ALDH in vitro. a MCF-7 cells were treated for 72 h with TCDD 10 nM and DMBA 5 μM in the presence and absence of AhR/CYP1A1 inhibitor, α-NF 10 μM. Pelleted cells were then incubated with ALDH substrate in the presence and absence of DEAB, ALDH inhibitor. Thereafter, percentage of ALDH+ cells were determined by flow cytometry. b Duplicate reactions were performed for each experiment and the values represent mean of fold change ± SEM (n = 6). *; p < 0.05 compared to control. #; p < 0.05 compared to corresponding treatment in the absence of α-NFBack to article page