Fig. 7

Effect of Wnt and Notch pathways inhibitors on TCDD-increased ALDH. a MCF-7 cells were treated for 72 h with TCDD 10 nM in the presence and absence of Wnt inhibitor (XAV-939, 5 μM) or Notch inhibitor (FLi 06, 5 μM). MCF-7 cell were then harvested, pelleted, and then incubated with ALDH substrate in the presence and absence of DEAB, ALDH inhibitor. Thereafter, percentage of ALDH+ cells were determined by flow cytometry. b MCF-7 cells treated with TCDD 10 nM were stained with primary antibodies against β-Catenin, Wnt transcription factor and ICN-1, Notch transcription factor followed by secondary antibodies. Thereafter, β-Catenin and ICN-1 proteins localization was conducted by immunofloursene assay. Each sample was stained in triplicate for each antibody