Fig. 6

TUG1 regulated LIMK2b expression by binding with EZH2. a Expression of LIMK2b in SCLC FFPE tissues by qRT-PCR. b The correlation of LIMK2b and TUG1 expression in FFPE tissues. c RIP experiments were performed in H69AR cells and the coprecipitated RNA was subjected to qRT-PCR for TUG1. The fold enrichment of TUG1 in LIMK2b RIP is relative to its matching IgG control. d e qRT-PCR and western blot analysis for LIMK2b in H446DDP, H69AR cells inhibited EZH2 with EI1 or GSK343 or transfected with siRNA targeting EZH2 (si-NC, si-EZH2 1* and si-EZH2 2*). f ChIp-qPCR of EZH2 occupancy and H3K27me3 binding in the promoters of LIMK2b in H69AR cells treated with siNC or si-TUG1; IgG as a negative control. *P < 0.05, **P < 0.001