Fig. 6

miR-4775 regulates the TGFβ pathway by directly repressing Smad7. a Potential miR-4775 binding sites in the Smad7 3′UTR and mutated miR-4775 binding sites in the Smad7 3′UTR. b Smad7 mRNA changes upon miR-4775 overexpression or downregulation (**p < 0.01;***p < 0.001). c–d Effects of miR-4775 overexpression or knock down on luciferase dual reporter activity with the wild-type and mutated Smad7 3′UTR. e p3TP and f SBE4 reporter assay to analyze the roles of miR-4775 overexpression or knock down on activation of TGFβ signaling. g Western blot analyses of Smad7/TGFβ pathway proteins after upregulation or downregulation of miR-4775 expression. h Effects of Smad7 overexpression on the protein levels of TGFβ pathway-associated markers in miR-4775-overexpressing and control cells. i Effects of decreases in Smad7 on the protein levels of TGFβ pathway-associated markers in cells knocked down for miR-4775 and the corresponding control group