Fig. 3

CD73 knockdown increased the sensitivity of NSCLC cells to gefitinib and lapatinib. a The expression of proteins in the CD73 and EGFR signaling pathways was detected in the stable A549 and H226 cell lines. Our data showed that p-EGFR and p-AKT expression is significantly decreased in CD73-silenced cell lines compared with the control cells. b After serum starvation for 24 h, CD73-silenced A549 and H226 cells were treated with or without EGF (50 ng/ml) for 30 min. The expression levels of CD73, p-EGFR, EGFR, p-AKT, AKT, p-ERK and ERK were analyzed by western blot analysis. c–f CD73 knockdown increased the sensitivity of NSCLC cells to gefitinib and lapatinib. The A549 and H226 cell lines were transfected with 10 μM gefitinib and 20 μM lapatinib for 48 h, respectively. After the aforementioned treatments, cell viability was assessed using CCK-8 assays. The data shown represent the mean ± SD values of four replicate experiments. All the data were obtained from three independent experiments and are shown as the mean ± SD values. ^** P 0.01; ^*** P < 0.001