Fig. 5

Dclk1 knockdown in the Apc ^min/+ mice reduced the stemness and pro-survival signaling of Dclk1⁺ cells. a Enteroids formation of isolated Dclk1⁺ cells (100 cell per well) from the small intestines of Apc ^Min/+ mice treated with siDclk1-NPs and siScramble-NPs. b Stacked bar and c line graph represent the quantification of the number of enteroids formed and spheroid volume from the isolated Dclk1⁺ cells of Apc ^Min/+ mice. d mRNA and protein expression of Dclk1, Bmi1, Lgr5, and Msi1 in the isolated IECs of Apc ^Min/+ mice treated with siDclk1-NPs and siScramble-NPs. e & f mRNA and protein expression of pro-survival signaling and their downstream targets in the isolated IECs of Apc ^Min/+ mice treated with siDclk1-NPs and siScramble-NPs. All quantitative data are expressed as means ± SD of a minimum of three independent experiments. P values of <0.05 = *, <0.01 = **, and 0.001 = *** were considered statistically significant