Fig. 5

HOXC8 expression negatively regulates CSC and chemoresistance. a Representative FACS profiles of cells transduced with pSIN-HOXC8 or pSIN empty control vector and quantification of CSC and non-CSC populations stained with CD44-APC and CD244-FITC. Results were analysed by comparing levels of CD44⁺/CD24⁻, CD44⁺/CD24⁺, CD44⁻/CD24⁺ cells in pSIN-HOXC8 transduced cells compared to control vector for each cell line (n = 3). Statistical analysis was performed by Two-way ANOVA followed by Bonferroni’s multiple comparisons test. **P < 0.01, ***P < 0.001. b Expression of CD24 and ALDH1A1 in Control and HOXC8-overexpressing cells. Gene expression was measured by TaqMan® qRT-PCR. Results are presented as relative fold expression relative to RPLP0 and control (pSIN vector) used as calibrator (n = 3). Relative fold expression levels were analysed by Unpaired Student’s t-test. **P < 0.01, ***P < 0.001. c Percentage of CD44⁺/CD24^low/- cells in cell lines treated with 10nM Paclitaxel, 500 μM 5-FU and 500nM Doxorubicin for 5 days as measured by FACS analysis. Results were analysed by comparing levels of CD44⁺/CD24^low/- cells in untreated versus treated cells by One-way ANOVA followed by Bonferroni’s multiple comparisons test for each cell line (n = 3). *P < 0.05. d Percentage of CD44⁺/CD24^low/- cells in cell lines treated with 10nM Paclitaxel, 500 μM 5-FU and 500nM Doxorubicin for 5 days as measured by FACS analysis. Results were analysed by comparing levels of CD44⁺/CD24^low/- cells in pSIN-HOXC8 or pSIN empty control vector transduced cells by Unpaired Student’s t-test for each cell line (n = 3). **P < 0.01