Fig. 6

HOXC8 expression reduced CSC self-renewal and anchorage independent cell growth. a Mammospheres morphology and number obtained by self-renewal of cell overexpressing HOXC8 or control vector after 4 generations. Mammospheres numbers were compared by Unpaired Student’s t-test for each cell line (n = 4). *P < 0.05, ***P < 0.001. Only spheres ≥ 100 μM were counted, images were taken at 10X magnification. b Second generation mammospheres obtained after labelling with the PKH26 dye and sorting of the PKH26 ^{positive/high} population from first generation mammospheres. Results show the morphology of mammospheres overexpressing HOXC8 or control vector and total mammospheres cell number. Mammospheres numbers were compared by Unaired Student’s t-test (n = 3). **P < 0.01. Only spheres ≥ 100 μM were counted, images were taken at 10X magnification. c Colonies of cells overexpressing HOXC8 or control vector grown in soft agar for 2 weeks. The number of colonies were calculated by counting 10 fields of view. Results were analysed by Unpaired Student’s t-test for each cell line (n = 3). **P < 0.01, ***P < 0.001. Images were taken at 10X magnification