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Fig. 3 | Molecular Cancer

Fig. 3

From: Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation

Fig. 3

Doxycycline (Dox) significantly decreased LPA and TNFα induced IL-6 mRNA expression in breast cancer cells. Cells were serum starved for 14 h in DMEM/0.1% BSA and then followed with LPA (1 μM) or TNFα (20 ng/ml) stimulation. For Dox-treated cells, 5 μg/ml of Dox was included during serum starvation and stimulation. a. Time course of LPA induced IL-6 expression in 4T1 cells and the inhibition by Dox. b. Time course of TNFα induced IL-6 expression in 4T1 cells and the inhibition by Dox. c. Time course of LPA induced IL-6 expression in MDA-MB-231 cells and the inhibition by Dox. d. Time course of TNFα induced IL-6 expression in MDA-MB-231 cells and the inhibition by Dox. * p < 0.05 relative to control. Results are means ± SEM from three independent experiments. Results were analyzed by ANOVA with an SNK posthoc test

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Molecular Cancer

ISSN: 1476-4598