Fig. 4
From: A loss of host-derived MMP-7 promotes myeloma growth and osteolytic bone disease in vivo
MMP-7 has limited effects on MMP-7 viability. a MMP-7 mRNA was measured in 2T3 osteoblasts and 5TGM1 myeloma cells. b TIMP-1 mRNA was measured in 2T3 osteoblasts, 14M1 myeloma-associated bone marrow stromal cells, ST2 bone marrow stromal cells and 5TGM1 myeloma cells. Treatment of 5TGM1 (c) or RPMI 8226 (d) myeloma cells with recombinant MMP-7 had limited effects on cell viability (n = 3). e Recombinant MMP7 can cleave murine galectin-3 into distinct fragments. 100 ng galectin-3 was incubated with increasing concentrations of MMP-7 (10–100 ng) and cleavage fragments detected by silver staining. f Over-expression of MMP-7 in 2 T3 osteoblasts decreased myeloma cell viability in a coculture of myeloma cells and osteoblasts (n = 3). Data represent mean ± SEM. Statistical analysis by one-way ANOVA and Tukey-Kramer posthoc test