Skip to main content

Table 1 Principal data regarding the relationship of EMT with PCSCs

From: The epithelial to mesenchymal transition (EMT) and cancer stem cells: implication for treatment resistance in pancreatic cancer

Experimental approach Molecular characteristics of PCSCs References
Short hairpin RNA (shRNA)-mediated ZEB1-knockdown in the two cells with the highest levels of ZEB1(Panc–1 and MiaPaCa–2) Reduction of CD24+/CD44+ subpopulation, reduced sphere formation in the two cancer cell lines and sphere numbers in subsequent generations decreased expression of stem cell factors such as Sox2, Bmi1 and p63 [96]
CD133 overexpression in Mia PaCa-2 cell Increased mRNA expression of several EMT-associated genes: SNAI1, ZEB1, Vimentin, CDH2 and MMP9. CD133hi-MIA cells show a more fibroblast-like morphology [97]
Silenced Snail in Panc-1 cells A significant decrease in the ALDHhigh population, reduction initial formation of spheres and sphere numbers in subsequent generations. [98]
Nestin shRNA in PANC-1 cell and nestin-overexpressing in MiaPaCa-2 cell Expression of mesenchymal markers, acquisition of invasive properties and high motility/opposite effects [99]
Isolate the SP cell fraction (side population, a cancer stem cell enriched fraction from Panc-1,KP-1NL and Capan-2 cell lines), incubate SP cells in the presence of TGF-β Production of cells with mesenchymal-like morphology,alteration such as reduction of E- cadherin mRNA and induction of Snail mRNA and (MMP)-2 mRNA [52]