Fig. 3

Downregulation of GPR87 decreases the aggressiveness of pancreatic cancer cells. a. Western blot analysis of GPR87 expression in PANC-1 and AsPC-1 cells following GPR87 silencing by RNAi. α-tubulin was used as a loading control. b. Representative images (left panel) and quantification (right panel) of colonies in an anchorage-independent growth assay. Colonies larger than 0.1 mm in diameter were scored. c. Representative micrographs (left panel) and quantification (right panel) of BrdU labeling in cells transfected with GPR87-RNAi or an RNAi-vector. d. Representative images (left panel) and quantification (right panel) of HUVECs cultured on matrigel-coated plates with conditioned medium from control and GPR87-RNAi pancreatic cancer cells. e. Representative images of CAM blood vessels stimulated with conditioned medium from the indicated cells. f. Representative images (upper panel) and quantification (lower panel) of invaded cells analyzed using a transwell matrix penetration assay. g. Representative images (left panel) and quantification (right panel) of Annexin V-FITC and PI staining of indicated cells treated with gemcitabine (1 μM) for 24 h. Each bar represents the mean ± SD of three independent experiments. * p < 0.05