Fig. 6

The CXCL5/CXCR2 axis regulates CRC cell invasion through the AKT/GSK3β/β-catenin/MMP7 pathway. a & b CXCL5 promotes CRC cell invasion in a CXCR2-dependent manner in HCT116. Inhibition of CXCL5 reverses this process in a CXCR2-dependent manner in SW480. Scale, 200 μm. Data are presented as the mean ± SD. c Immunofluorescence images show that CXCL5 promotes translocation of β-catenin in a CXCR2-dependent manner in HCT116. Inhibition of CXCL5 reverses this process in a CXCR2-dependent manner in SW480. Scale, 50 μm. d The inhibitory effects of β-catenin- shRNA on β-catenin in HCT116^CXCL5 and SW480 cells. Scale, 50 μm. e & f Inhibition of β-catenin decreases the invasion of CRC cells. Scale, 200 μm. Data are presented as the mean ± SD. g & h CXCL5/CXCR2 enhances the expression of MMP7 through activation of the AKT/GSK3β/β-catenin pathway. i & j Inhibition of the AKT pathway (LY294002) instead of the ERK pathway (U0126) reverses the activation of GSK3β/β-catenin and upregulates the expression of MMP7. *P < 0.05, **P < 0.01, ***P < 0.001