Fig. 5
PinX1 inhibit xenograft tumor growth in vivo and target moleculars in NSCLC cells cycle transition. a The six proteins, P15INK4b, CDK4, CyclinD1, CyclinD2, Rb, and the p-Rb(P-Ser608) were examined in PinX1-siRNA A549 cells compared with that of empty vector by using Cell Cycle Antibody Array (Full moon BioSystems) (left panel). The relative fluorescence intensity fold change (A549 PinX1 shRNA/Mock) was displayed using histogram (right panel). b The tumor volume of xenografts was measured calipers every 4 days for 40–46 days. The values represent mean tumor volume ± SE. *, compare with A549 group (P < 0.05), ** compare with SK-MES-1 group (P < 0.05). c Downregulation of P15INK4b, Rb and upregulation of CDK4, CyclinD1, p-Rb(p-Ser608) were observed in PinX1-slienced A549 cells by western blotting. d The expression of and BMP5 were detected in PinX1-slienced A549 cells and SK-MES-1 PinX1 cells